References for Tissue Clearing Protocols

Make it *clearly* visible with our references and imaging systems by ZEISS

Knowledge

Mouse brain, optically cleared with CLARITY, courtesy of Bergan&Dulac, Harvard University
Mouse brain, optically cleared with CLARITY, courtesy of Bergan&Dulac, Harvard University

 

Tissue clearing allows you to image deep into highly scattering biological samples (e.g. brains, embryos, organs, spheroids). Tissue clearing is a chemical procedure which replaces light-scattering structures in the sample by molecules which have the same refractive index as the surrounding sample material. This results in enhanced optical penetration to capture fluorescent signals inside of tissues almost without any artifacts caused by scatter.

 

Methodology of Tissue Clearing Techniques. Richardson&Lichtman, Cell 2015
Methodology of Tissue Clearing Techniques. Richardson&Lichtman, Cell 2015

 

A recent review by Doug Richardson and Jeff Lichtman from the Harvard Center for Biological Imaging (HCBI) gives an excellent overview of the available clearing methods. The HCBI has been working closely with ZEISS Microscopy, being the first research lab to rapidly image cleared tissue with Lightsheet Z.1. Together with arivis Vision4D, a software solution for visualization and analysis of huge imaging data sets that was recognized by a Microsoft Life Sciences Innovation Award in 2014, you acquire and visualize even large 3D datasets in a short period of time.

 

The HCBI Tissue Clearing Workshop 2015
The HCBI Tissue Clearing Workshop 2015

 

The HCBI hosted a 3-day workshop this fall covering all aspects of tissue clearing.  Each day consisted of morning lectures, followed by hands on wet-lab or microscopy experimentation in the afternoon.  In addition, evening social events allowed time for networking with colleagues and ZEISS specialists.  Attendees were introduced to both aqueous- and solvent-based clearing procedures. The following imaging techniques were highlighted: linear point-scanning confocal, 2-photon, structured illumination (ApoTome), light sheet fluorescence, and Airyscan microscopy.

The Tissue Clearing Workshop 2015 at Harvard HCBI has finished successful. A big thanks to all participants!
The Tissue Clearing Workshop 2015 at Harvard HCBI has finished successful. A big thanks to all participants!



References List:

 

See also: How-to: Mount Cleared Samples for ZEISS Lightsheet Z.1

 

Tags: Confocal Microscopy, Light Microscopy, Light sheet Microscopy

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