Make it *clearly* visible with our references and imaging systems by ZEISS
Tissue clearing allows you to image deep into highly scattering biological samples (e.g. brains, embryos, organs, spheroids). Tissue clearing is a chemical procedure which replaces light-scattering structures in the sample by molecules which have the same refractive index as the surrounding sample material. This results in enhanced optical penetration to capture fluorescent signals inside of tissues almost without any artifacts caused by scatter.
A recent review by Doug Richardson and Jeff Lichtman from the Harvard Center for Biological Imaging (HCBI) gives an excellent overview of the available clearing methods. The HCBI has been working closely with ZEISS Microscopy, being the first research lab to rapidly image cleared tissue with Lightsheet Z.1. Together with arivis Vision4D, a software solution for visualization and analysis of huge imaging data sets that was recognized by a Microsoft Life Sciences Innovation Award in 2014, you acquire and visualize even large 3D datasets in a short period of time.
- Cleared tissue imaging at the Harvard Center for Biological Imaging
- Review: Clarifying Tissue Clearing (Richardson&Lichtman, Cell 2015)
- ZEISS Lab Poster Download: Clearing Methods in Microscopy
- CLARITY Resources
- SeeDB Resources
- CUBIC (Tainaka et al, Cell 2014)
- PACT (Yang et al, Cell 2014)
- Scale (Hama et al, Nature 2011)
- ScaleS (Hama et al., Nature Neuroscience 2015)
- FRUIT (Hou et al, Frontiers in Neuroanatomy 2015)
- ClearSee for plant tissue (Kurihara et al., Development 2015)