The CVR is embedded within the Institute of Infection, Immunity and Inflammation at the University of Glasgow, which provides excellent research opportunities to investigate virus-host interactions and immune response to virus infection. A defining feature of the CVR is its breadth of expertise ranging from molecular virology to in vivo pathogenesis, virus-cell interactions, viral immunology, viral ecology, viral oncology, clinical and veterinary virology, viral diagnostics, virus epidemiology, mathematical modelling, virus genomics & bioinformatics.
We profiled four scientists who use light microscopy to highlight some of the research being performed at the CVR.
She works on trying to understand how the bacterium Wolbachia blocks the replication of clinically important arboviruses, such as dengue. The primary vector for dengue, Aedes aegypti (mosquito), does not naturally harbor Wolbachia, which is found in ~66% of insects. However, the Sinkins’ group is able to transinfect the bacterium into the mosquito and have shown a 80% reduction in dengue cases in their Malaysian release site, compared to control sites.
To understand how Wolbachia blocks viruses, Dr. Rainey and the group uses a range of techniques to look at what is going on at a cellular level. They infect cells and mosquitoes with different arboviruses (arthropod/insect borne viruses) and then use microscopy to see how Wolbachia affects the replication complexes, where the virus multiplies and how both affect the structures inside the cell. To do this they use both confocal and light sheet microscopy. They use live samples, fixed samples, and cells.
One of the things they have found is that cholesterol trafficking is important for blocking viruses when Wolbachia is present. They also use fluorescence in situ hybridization to look at density and location of the Wolbachia within tissues and cells.