New Light Sheet Microscope for Multiview Imaging of Large Specimens

ZEISS Lightsheet 7 works with both whole living organisms and large, cleared samples

Mouse kidney cleared with iDISCO protocol and imaged in ethyl cinnamate with ZEISS Lightsheet 7 detection optics 5× / 0.16 foc and Clr 20× / 1.0 nd = 1.53 (insert). The mouse was perfused with DyLight 594 conjugated tomato lectin to visualize vasculature and glomeruli (red). In green: auto-fluorescence to visualize tissue anatomy. 3D whole organ imaging and computational image analysis of glomerular size and number helps to gain a better understanding of the mechanisms of diverse kidney diseases, e.g. diabetic nephropathy. © Sample courtesy of U. Roostalu, Denmark

Light sheet fluorescence microscopy (LSFM) with its unique illumination principle allows fast and gentle imaging of whole living model organisms, tissues, and developing cells. The high stability of ZEISS Lightsheet 7 enables researchers to observe living samples over extended periods of time – even days – with less phototoxicity than ever before. The new light sheet microscope can also be used to image very large optically cleared specimens in toto, and with subcellular resolution. The dedicated optics, sample chambers, and sample holders of ZEISS Lightsheet 7 can be adjusted to the refractive index of the chosen clearing method to observe large samples, even whole mouse brains.

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