Application note details experimental workflows using images captured with automated microscopy
The use of 3D cell culture models has emerged as a fundamental tool throughout various life science disciplines including tumor and translational biology, developmental and basic cell biology.
However, use of microscopy with 3D cell cultures bring new experimental challenges, including:
- Previously common analytics become more complicated when adding a third dimension. Some examples are: tracing of individual events, marker-based signal quantification, large throughputs in time-series or end-point measurements.
- Three-dimensional cell cultures are more fragile, making identification and quantification of cellular and compartmental events difficult.
- Special supports with thick well bottoms, non-planar u-bottom shapes or very high skirt heights are often necessary, which are challenging for most optical devices as well as automation routines and analysis algorithms.
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