Comparing Airyscan technology to conventional confocal imaging in live cell imaging
The recently published peer-reviewed paper “Exploring the Potential of Airyscan Microscopy for Live Cell Imaging” by Kseniya Korobchevskaya, B. Christoffer Lagerholm, Huw Colin-York and Marco Fritzsche from the University of Oxford, UK highlights the significant improvement of resolution and signal-to-noise at the same time of ZEISS Airyscan compared to conventional confocal imaging techniques. The article was published as part of the Special Issue Superresolution Optical Microscopy.
“Biomedical research demands non-invasive and ultra-sensitive imaging techniques. Especially, our laboratory for Biophysical immunology at the MRC Human Immunology Unit and Kennedy Institute for Rheumatology at the University of Oxford relies on state-of-the-art imaging technology with extended spatial and temporal resolution as offered by the novel ZEISS Airyscan technology. In our recent paper, we demonstrate how Airyscan imaging successfully bridges the gap between conventional confocal and super-resolution microscopy”, says Marco Fritzsche.
Abstract: Biological research increasingly demands the use of non-invasive and ultra-sensitive imaging techniques. The Airyscan technology was recently developed to bridge the gap between conventional confocal and super-resolution microscopy. This technique combines confocal imaging with a 0.2 Airy Unit pinhole, deconvolution and the pixel-reassignment principle in order to enhance both the spatial resolution and signal-to-noise-ratio without increasing the excitation power and acquisition time. Here, we present a detailed study evaluating the performance of Airyscan as compared to confocal microscopy by imaging a variety of reference samples and biological specimens with different acquisition and processing parameters. We found that the processed Airyscan images at default deconvolution settings have a spatial resolution similar to that of conventional confocal imaging with a pinhole setting of 0.2 Airy Units, but with a significantly improved signal-to-noise-ratio. Further gains in the spatial resolution could be achieved by the use of enhanced deconvolution filter settings, but at a steady loss in the signal-to-noise ratio, which at more extreme settings resulted in significant data loss and image distortion.
The authors of this paper focused on the superresolution aspect. To learn more about the enhanced sensitivity and speed of ZEISS Airyscan read this free paper.
Watch this video and understand how ZEISS Airyscan works: